Question

ELISA test is used to

• A. Separate viral RNA
• B. Purify proteins
• C. Isolate DNA sequences
• D. Identify specific proteins

Answer 1

The correct option is D Identify specific proteins

ELISA is also known as enzyme-linked immunosorbent assay. It is used to detect the presence of proteinaceous antigen and antibody in a given sample. The sample containing a protein is attached to a surface. Antibody specific to the protein is added which agglutinates with it. The antibody is linked to an enzyme relevant to which enzyme's substrate is added. The enzyme acts on the substrate and shows detectable colour change which is an indicator of the presence of antigen in given sample.

So, the correct answer is option D.