The correct option is A Removal of anther
Emasculation involves the removal of stamens from bisexual flowers of the female parents in order to avoid self-pollination in these flowers. It is done before the anthers are mature. If in a condition the female parent bears bisexual flowers, then elimination of anthers from the flower bud before the anther dehisces by means of a pair of forceps (a large instrument with broad blades) is necessary. And this step is known as emasculation. Thus, the correct answer is option A.