Question

Embryoids formed in tissue culture from pollen grain are due to

• A. Test tube culture
• B. Cellular totipotency
• C. Organogenesis
• D. Double fertilisation

Answer 1

Answer: (B)

Cellular totipotency

Embryoids formed in tissue culture from pollen grain is due to cellular totipotency of the callus developed by the explant. The explant is surface sterilized and is cultured on the nutrient media which develops into a callus. Callus has totipotent cell which further forms embryoids. Embryoid is a three-dimensional structure which contains the pleuripotent cells. It has the ability to differentiate when cultured on artificial media. It is a non-zygotic embryo formed in vitro culture which when cultured on the nutrient media develops into developing embryo and it develops into a plantlet.

So, the correct answer is option B.