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Question

Enzyme that is central to the PCR technology is

A
Taq polymerase
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B
Polymerase
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C
Helicase
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D
Reverse transcriptase
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Solution

The correct option is A Taq polymerase

The polymerase chain reaction is a technique by which small samples of DNA can be quickly amplified. The repeated amplification is achieved by the use of thermostable DNA polymerase, that is Taq polymerase which remain active during the high temperature induced denaturation of double-stranded DNA. Taq polymerase is isolated from the Thermophilus aquaticus, a bacteria that survives in high temperature enviornments such as hot springs.


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