Question

Enzymes can be immobilized by

• A. Cross-linking enzyme molecules
• B. Covalently attaching to a solid support
• C. Entrapping them in gel
• D. All of the above

Answer 1

The correct option is D All of the above

There are four principal methods available for immobilizing enzymes as follows:

(a) Enzyme non-covalently adsorbed to an insoluble particle;

(b) Enzyme covalently attached to an insoluble particle;

(c) Enzyme entrapped within an insoluble particle by a cross-linked polymer;

(d) Enzyme confined within a semi-permeable membrane.