Question

Enzymes necessary for recombinant DNA technology are

• A. Endonucleases and polymerases
• B. Restriction endonucleases and ligases
• C. Peptidases and ligases
• D. Restriction endonucleases and topoisomerases

Answer 1

The correct option is B Restriction endonucleases and ligases

Recombinant DNA technology is the method of joining two or more DNA molecules to create a hybrid. This technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence. DNA fragments generated by digestion with a restriction endonuclease can be joined together again by the enzyme ligase.

So the correct answer is option B.