Enzymes used in breaking DNA at specific sites are
A
DNA-ases
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B
Endonucleases
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C
Restriction endonucleases
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D
Exonucleases
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Solution
The correct option is C Restriction endonucleases
Restriction enzymes are used in genetic engineering for gene manipulation. It cuts DNA base pairs at specific sites. They cut the base pairs within the molecule so they are named as an endonuclease. 3000 restriction enzyme has been discovered till now. They are known as molecular scissors as they are used to cut a single gene from a larger piece of DNA. The restriction enzyme Eco RI, Bam II and Hind Ill are used in recombinant DNA technology to produce cuts in vector and other DNA molecules to obtain chimeric DNA.