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Bacterial Synthesis of Insulin (Human)

Escherichia c...

Question

Escherichia coli having plasmids for production of human insulin is cultured in medium containing __________.

A

Glucose

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B

Maltose

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C

Lactose

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D

Sucrose

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Solution

The correct option is C Lactose
Escherichia coli, the most studied microorganism, has been found to possess a variety of plasmid types including those associated with the production of human insulin.
It is cultured in a medium containing lactose. If the lactose is absent then the repressor will not bind the operator allowing transcription of the lac operon genes. The repressor will bind the operator allowing transcription of the lac operon genes.
So, option C is the correct option.

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Q. Read the following statement having two blanks A and B.
In ”____A____, ____B____” an American Company prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of Escherichia coli to produce insulin chains.”
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Q. Some of the steps involved in the production of humulin are given below. Choose the correct sequence
(i) Synthesis of insulin gene artificially
(ii) Culturing recombinant E. coli in bioreactors
(iii) Purification of humulin
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iii. Purification of human insulin
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