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Test for Oxygen

Evidence for ...

Question

Evidence for the liberation of O $_{2}$ from water during photosynthesis comes from

Release of

^{18}

O if water contains the same

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Isolated chloroplast supplied with reducing agent like potassium ferrocyanide evolves O

_{2}

even in absence of CO

_{2}

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Photosynthetic bacteria do not Iiberate O

_{2}

and they use H

_{2}

S for obtaining reducing power

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All of the above

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Solution

The correct option is A All of the above
A. Ruben and Kamen used the radioactive isotope of oxygen ( $^{1}$ $^{8}$ O) in water and found that O $_{2}$ liberated in the process was isotopically labelled. On the contrary, when $^{1}$ $^{8}$ O in CO $_{2}$ was used, the evolved O $_{2}$ did not show radioactivity.
B. Hill and Bendall isolated chloroplast from leaves of Stellaria media and experimented in the absence of CO $_{2}$ .
$2 A + 2 H_{2} O \to 2 A H_{2} + O_{2}$
In the presence of Artificial Hill reagent Potassium ferricyanide and sunlight, this experiment is performed.
C. Van Niel observed that during bacterial photosynthesis hydrogen sulphide was broken down to hydrogen and sulphur. The hydrogen reduces carbon dioxide to synthesize carbohydrate and sulphur is accumulated as a waste product.
Hence, the correct answer is 'All the above'.

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the reducing agent is –

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