Question

Explain an activity to observe a prepared temporary mount of onion peel.

Answer 1

Explanation :

1. A small sample of tissue can be taken from the onion peel to make a slide and stained with a suitable dye such as safranin and be viewed under the microscope.
2. Glycerine has a high refractive index and thus can be used in the slide for better vision.

Materials required:

1. Forceps
2. Needle
3. Onion
4. Slide
5. Coverslip
6. Glycerin
7. Safranin
8. Microscope

Experiment:

1. Peel off a thin peel from the onion bulb with the help of forceps and a needle.
2. Place the peel on a slide.
3. Put a drop of glycerin on it and also put a drop of orange safranin solution on it.
4. Cover it carefully with the help of a needle with a coverslip to avoid any air bubbles.
5. Observe it under a microscope of appropriate power.