CO2 fixation occurs by means of the Calvin cycle reactions, that occur in the stroma of the chloroplast. These reactions occur in three stages: fixation, reduction, and regeneration (shown in the image). During CO2 fixation enzyme RuBisCO catalyzes a reaction between CO2 and RuBP, forming a six-carbon compound that is immediately converted into two three-carbon compounds (3-PGA). This process is called carbon fixation because CO2 is “fixed” from its inorganic form into organic molecules.