Explain how to find whether an E.coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it.

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Solution

The recombinant can be selected out from the non-recombinants by plating the transformants on ampicillin-containing medium. The transformants will grow in it, while the non-transformants will not grow.

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Q. (a) Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it.
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