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Question

Explain the following terms in connection with the concept of lac operon in E.coli (a) structural genes b) operon c) cistron (d) polycistron and (e) repressor.

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Solution

(A) Structural genes are the segments of DNA, which carry codes for the synthesis of proteins. These genes determine the primary structure of a polypeptide chain by having the coding sequence of amino acids in them. For eg, the lac operon of E.coli contains three structural genes, i.e, z, y and a.
(B) An operon is a functioning unit of DNA containing a cluster of genes under the control of a single promoter.
(C) Cistron is an alternative term to the gene which is involved in producing a polypeptide chain.
(D) Polycistron is a type of messenger RNA that encode more than one polypeptide.
(E) The repressor is a DNA or RNA binding proteins that inhibit the expression of one or more genes by binding to the operator.

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