Question

Explain the process and the principle behind the LAMP assay in detail and also in simple way, Also, the equipment needed to carry out the entire assay ?

Answer 1

1. Loop mediated isothermal amplification (LAMP) is a single tube technique for the amplification of DNA
2. First Primer binds and DNA polymerase generates new strand
3. Next Primer binds and displaces the new DNA
4. DNA polymerase generates new strand in the place of the previously created one, which dissociates Note: the teal denotes the same DNA sequence as the pink, just another copy of it
5. Now a similar thing happens with the backward primers.
6. Note: the newly created strand is now the template strand
7. The strands then separate and the primers that were coded on the ends anneal to themselves, creating the start of the LOOP
8. The process then repeats, generating longer and longer chains of the target gene linked together by the loop primers
9. The process continues to repeat itself generating longer chains of the target gene, linked together by the loop primer

First Primer binds and DNA polymerase generates new strand

Next Primer binds and displaces the new DNA. DNA polymerase generates new strand in the place of the previously created one, which dissociates
Note : The teal denotes the same DNA sequence as the pink, just another copy of it

Equipment name : Microfluidic LAMP