Question

explain the role of RNA polymerase in transcription in bacteria ?

Answer 1

Role of RNA Polymerase in transcription of bacteria:
RNA polymerase is an enzyme that catalyses in only one direction i.e., 5' to 3' and so uses the strand with polarity 3'→ 5' as a template or template Strand. It binds with the promoter to initiate the process of transcription.Association with initiation factors ( sigma factor)alters the specificity of RNA polymerase to initiate the transcription. During elongation, RNA polymerase uses nucleotide triphosphate as substrate, and polymerisation occurs according to complementarity.
At the time of termination, the termination factor (P) alters the specificity of RNA polymerase to terminate the transcription.
As the RNA polymerase proceeds to perform elongation, a short stretch of RNA remains bound to the enzyme. As the enzyme reaches the termination region, this nascent RNA falls off and transcription is terminated.