Tissue culture is a process which involves the growth of animal tissues or cells separate from the organism. This is typically facilitated by the use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. It involves exposing plant tissues to a specific regimen of nutrients, hormones, and light under sterile, in-vitro conditions to produce new plants, each one a clone of the original mother plant, over a very short period of time.The term “tissue culture” was coined by the American pathologist Montrose Thomas Burrows.