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Question

Explant required for virus free culture is

A
root
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B
shoot tip
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C
leaf
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D
leaf and root
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Solution

The correct option is B shoot tip
Explants are small pieces of plant parts or tissues that are aseptically cut and used to initiate a culture in a nutrient medium. Generally plant shoot apical meristems are virus free. This is because the spread of the virus in the plant tissue does not keep up (is slow) vis a vis the fast meristem growth if it is actively producing new leaves/flowers. The meristem grows into a virus-free shoot that is rooted to get a virus-free plant.

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