The correct option is C After entry of sperm but before fertilization
Meiotic division of the secondary oocyte/ovum occurs after the entry of sperm into the ovum. This entry causes the breakdown of MPF (Metaphase promoting factor) and initiates the anaphase complex, known as APC and also causes release of second polar body.
So, extrusion of second polar body occurs -after entry of sperm but before fertilization.