The egg fertilizin of a particular species reacts best with the sperm antifertilizin of the same species.This reaction is highly specific. Reactions between the species which are nearly related to each other are very much weaker and sometimes failure.
However, normal species specificity is overcome if various abnormal conditions are used. For example, if sperm
concentration, pH, age of gametes, and other variables are manipulated, the chance of cross-fertilization is greatly increased.