The correct option is C on
From among the given prepositions, option C "on" indicates the particular day or part of the day during which an event takes place. The context refers to the event of his brother being present on a particular Sunday. Thus, "on" is correct as it indicates the exact day on which the event referred to in the sentence takes place.
"In" expresses the situation of something that is or appears to be enclosed or surrounded by something else.
"By" indicates the agent performing the action.
"At" indicates the location or arrival in a particular place or position.
Thus, option C is correct.