The correct option is
D He is
The correct answer is Option D.
The speaker wants to know how old is the listener's brother.
Since brother is a masculine word, the corresponding pronoun will be HE. And the sentence is in the present tense, therefore the answer will also be in the present tense. Thus, HE IS is the correct answer.
Option D is the correct answer.
The remaining options are incorrect because:
Option A: THEY is plural while only a single person - BROTHER is referred to in the given sentence.
Option B: I AM would be the correct answer if the speaker was asking the listener's age.
Option C: IT IS is used while referring to any thing not person.
Option D is thus the correct answer.