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Question

Find iso-electric point of amino acid :
862784_1a926bd4c01f4e58a29539dd41233db1.png

A
3.3
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B
5.9
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C
9.6
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D
11.8
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Solution

The correct option is A 3.3
CH3CH|NH3COOH COOHPka1=2.2
N+3PKa2=4.4
Calculation of amino acid,
This step is refers to the image.
At low pH all protonated.
P.I of amino acid=12(pka1+pka2)
=12(2.2+4.4)=12×6.6=3.3

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