Find iso-electric point of amino acid :
In an amino acid, the carboxyl group ionizes at pKa1=2.34 and ammonium ion at pKa2=9.6. The isoelectric point of the amino acid is at pH.
An important spectral emission line has a wavelength of 21 cm. The corresponding photon energy is (h=6.62×10−34Js; c=3×108m/s)