Question

Find the mismatch

• A. pBR322 - artificially synthesised plasmid
• B. YAC - Host organism
• C. BAC - bacterial artificial chromosome
• D. pUC19 - artificially synthesised plasmid

Answer 1

The correct option is B YAC - Host organism

YAC is an abbreviation for yeast artificial chromosome. It is a vector which includes a DNA molecule obtained from yeast. It is used to clone large DNA fragments. It has a very high insert capacity.

Hence, YAC is not a host organism. In biotechnology, an organism into which the recombinant DNA is introduced is called a host organism.

pBR322 is an artificial cloning vector (plasmid) constructed by Bolivar and Rodriguez. It has two antibiotic resistance genes and several cloning sites.

BAC is an abbreviation for bacterial artificial chromosome obtained from bacteria, a prokaryote. It is used as a vector in biotechnology to carry the desired genes.

pUC19 is a genetically modified artificial plasmid which was created in the University of California. Thus, it was named pUC (p for plasmid, U for University and C for California).

YAC, BAC, pBR322 and pUC19 are vectors used to carry the gene of interest in genetic engineering.