The correct option is A Spongy mesophyll
C carbon fixation is a metabolic pathway for carbon fixation in photosynthesis, along with C and CAM metabolic pathways. In C4 plants, for example maize, PEP carboxylase fixes bicarbonate in the mesophyll cells of the leaf and the resulting four-carbon compound, malate, is shuttled into the bundle sheath cells where it releases CO for fixation by RuBisCo. So, first carbon fixation in C4 plants occurs in spongy mesophyll cells. Thus, option B is correct and other options are incorrect.