First enzyme to be isolated in pure crystalline from was
A.Zymase – enzyme involved in fermentation, first discovered by Edward Buchner.
B.Urease - catalyzes the hydrolysis of urea into ammonia and carbon dioxide. It is the enzyme first isolated by James Sumner.
C.Invertase - hat catalyzes the hydrolysis (breakdown) of sucrose (table sugar) into fructose and glucose.
D.Diastase - that catalyses the breakdown of starch into maltose.
So, the correct option is ‘Urease’.