Question

First strand of cDNA can be synthesized by

• A. Transaminase
• B. DNA ligase
• C. RNA dependent DNA polymerase
• D. DNA dependent DNA polymerase

Answer 1

The correct option is C RNA dependent DNA polymerase

In genetics, complementary DNA or cDNA is double-stranded DNA synthesized from a single stranded RNA. This single stranded RNA can either be mRNA ( messenger RNA) or microRNA template. cDNA is most often synthesized from mature mRNA using the enzyme reverse transcriptase or RNA dependent DNA polymerase where this operates on a single strand of mRNA, generating its complementary DNA based on the pairing of RNA base pairs.