The correct option is A Bacteria, fungi and protozoa
During the secondary treatment primary effluent is passed into large aeration tank where it is constantly agitated mechanically and air is pumped into it. This allows vigorous growth of useful aerobic microbes into flocs.
The biological floc is composed of bacteria, fungi and protozoa. Bacteria involve around 95% of the total microbial population of the activated sludge and they are essential for the degradation of organic substances in wastewater treatment systems.