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Question

Fluorescent microscope is used

A
To localize cell structure as chlorophyll stained with fluorochromes.
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B
To study living cells.
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C
To study smallest organelle.
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D
All of the above.
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Solution

The correct option is B To localize cell structure as chlorophyll stained with fluorochromes.
Fluorescent microscope is a modification of ultraviolet microscope which was made by Coons in 1945. The instrument uses long wave ultraviolet rays for illumination. It has complementary filters which allow the viewer to observe directly with the eyes. The microscope is useful in detecting those components which show autofluorescence eg. Chlorophylls, collagen fibrils, vitamin A etc.
So, the correct answer is 'To localize cell structure as chlorophyll stained with fluorochromes'.

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