Question

Following a severe accident, many charred-disfigured bodies are recovered from the site making the identification of the dead very difficult. Name and explain the technique that would help the authorities to establish the identity of the dead to be able to hand over the dead to their respective relatives.

Answer 1

The technique of DNA fingerprinting would help the authorities to establish the identity of the dead to be able to hand over the dead to their respective relatives.
Steps/procedure in DNA fingerprinting:
Extraction of DNA: using high speed refrigerated centrifuge.
Amplification: many copies are made using PCR
Restriction Digestion: using restriction enzymes DNA is cut into fragments.
Separation of DNA fragments: using electrophoresis agarose polymer gel.
Southern Blotting: Separated DNA sequences are transferred onto nitrocellulose or nylon membrane.
Hybridization: The nylon membrane exposed to radioactive probes.
Autoradiography: The dark bands develop at the probe site.