Question

For DNA fingerprinting to associate a blood sample with the criminal, what is minimally required

• A. One sample of DNA, PCR amplification, restriction enzymes, and gel electrophoresis
• B. One sample of DNA, restriction enzymes, a DNA synthesizer, and gel electrophoresis
• C. Two samples of DNA, PCR amplification, restriction enzymes, and gel electrophoresis
• D. Two samples of DNA, PCR amplification, restriction enzymes, and a DNA synthesizer

Answer 1

The correct option is C Two samples of DNA, PCR amplification, restriction enzymes, and gel electrophoresis

Two samples of DNA are required, one from the site of crime while another from the suspected criminal. These both samples of DNA are matched by DNA fingerprinting which involves identifying patterns of DNA known as a variable number of tandem repeats. PCR amplification is used to amplify DNA segments and restriction enzymes for cutting of DNA segments. Gel electrophoresis is used to separate these cut DNA segments based on their size.

So, the correct option is 'Two samples of DNA, PCR amplification, restriction enzymes, and gel electrophoresis'.