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Chromosomes

For karotype ...

Question

For karotype analysis, the cell division is paused at

A

Metaphase

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B

Prophase

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C

Anaphase

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D

Telophase

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Solution

The correct option is A Metaphase
Karyotyping is the process by which cytogenetist take photographs of chromosomes in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalies. Joe Hin Tjio was responsible for finding the approach of Karyotyping:
Using cells in tissue culture
Pretreating cells in a hypotonic solution, which swells them and spreads the chromosomes
Arresting mitosis in metaphase by a solution of colchicine
Squashing the preparation on the slide forcing the chromosomes into a single plane.
Cutting up a photomicrograph and arranging the result into an indisputable karyogram.

So, the correct answer is 'Metaphase'.

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