Question

For producing protoplasts from plant cells, which of the following are required?

• A. Amylase and pectinase
  
• B. Cellulase and proteinase
  
• C. Cellulase and pectinase
  
  
• D. Cellulase and amylase

Answer 1

The correct option is C Cellulase and pectinase



Protoplasts are cells which have had their cell wall removed, usually by digestion with enzymes. Cellulase enzymes digest the cellulose in plant cell walls while pectinase enzymes break down the pectin holding cells together. Once the cell wall has been removed the resulting protoplast is spherical in shape.
Digestion is usually carried out after incubation in an osmoticum (a solution of higher concentration than the cell contents which causes the cells to plasmolyse). This makes the cell walls easier to digest.