For the enzyme enolase, the substrate is

Succinic acid

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PGA

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PEP

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Fumaric acid

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Solution

The correct option is C PGA
Glycolysis converts glucose into two 3-carbon molecules called as pyruvate. The energy released during glycolysis is used to make ATP. Enolase is used to convert 2-phosphoglycerate (2PG) to phosphoenolpyruvate (PEP) in the 9th reaction of glycolysis. It is a reversible dehydration reaction.
Thus, the correct answer is option B.

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