For the preparation of agarose gel in electrophoresis, agarose is dissolved in
A
TAE buffer
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B
Distilled water
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C
Oil based emulsion
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D
Alcohol
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Solution
The correct option is A TAE buffer TAE is composed of a mixture of Tris base acetic acid and EDTA. Tris acetic acid component maintains the pH of the medium and also provides necessary ions for the conduction of electric current. EDTA component deactivates any DNase enzyme present in the medium thus leading to the smooth gel run.