For the same objective, what is the ratio of the least separation between two points to be distinguished by a microscope for light of 5000ËšA and electrons accelerated through 100V used as an illuminating substance ?
Given,
The voltage by which electrons are accelerated is 100V
The wavelength of given light is λ1=5000A∘
Now the limit of resolution of the microscope is given as
2dsinθ=λ
Hence,
2d1sinθ=λ1
2sinθ=λ1d1 ……. (1)
Now according to de Broglie wavelength equation
λ=12.27√VA∘
Hence,
λ2=12.27√100=1.227A∘
Also
2sinθ=λ2d2 …… (2)
Comparing equation (1) and (2)
λ2d2=λ1d1⇒d2d1=λ2λ1
d2d1=1.2275000⇒d2d1=0.00024
Therefore the ratio of least separation between two points to be distinguished by the microscope is 0.0002 .