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Reproduction in Bacteria

For transform...

Question

For transformation with recombinant DNA, the bacterial cells must first be made ‘competent’ which means

A

Increasing efficiency with which DNA enters the bacterium

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B

Ability to divide fast

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C

decreasing their metabolic reactions

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D

increasing their metabolic reactions

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Solution

The correct option is A Increasing efficiency with which DNA enters the bacterium
For transformation with recombinant DNA, the bacterial cells must first be made competent which means increasing the efficiency with which the DNA enters the bacterium.

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Similar questions

Q. Why DNA cannot pass through the cell membrane? Explain. How is a bacterial cell made "competent" to take up recombinant DNA from the medium?

Q. Assertion: The bacterial cells must first be made ‘competent’ to take up foreign DNA.
Reason: DNA cannot pass through cell membranes since it is a hydrophilic molecule.
Select the correct option.

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. Host cells are treated with chemical agents to increase their competency for taking in recombinant DNA during the production of transgenic organisms. Why is this step necessary?

Q. How many of the following statement(s) regarding insertional inactivation of lacZ gene are incorrect?

1. lacZ gene remains active in non-recombinant transformed bacteria.
2. X-gal gets hydrolysed and produces a blue product in non-recombinant transformed bacteria.
3. Bacterial colonies which are transformed with recombinant DNA appear white.
4. 𝛃-Galactosidase is produced in recombinant bacteria.
5. Bacterial colonies which are transformed with recombinant DNA appear blue.

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