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Question

GAATTC is recognition site of restriction endonuclease

A
Hind III
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B
EcoR I
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C
Bam I
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D
Hae III
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Solution

The correct option is B EcoR I
GAATTC is recognition site of restriction endonuclease EcoR I. EcoR I is the restriction enzyme, derived from Escherichia coli, strain R and is the first to be isolated. EcoR I recognizes and cuts at the specific restriction site 5'-GAATTC-3' and produces sticky ends. This is the common enzyme that is used as a tool for the restriction of the bacterial plasmid and desired genes in molecular biology techniques.

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