Question

GAATTC is recognition site of restriction endonuclease

• A. Hind III
• B. EcoR I
• C. Bam I
• D. Hae III

Answer 1

The correct option is B EcoR I

GAATTC is recognition site of restriction endonuclease EcoR I. EcoR I is the restriction enzyme, derived from Escherichia coli, strain R and is the first to be isolated. EcoR I recognizes and cuts at the specific restriction site 5'-GAATTC-3' and produces sticky ends. This is the common enzyme that is used as a tool for the restriction of the bacterial plasmid and desired genes in molecular biology techniques.