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Gel Electrophoresis

Gel electroph...

Question

Gel electrophoresis is used for:

A

Construction of recombinant DNA by joining with cloning vectors

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B

Isolation of DNA molecules

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C

Cutting of DNA into fragments

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D

Separation of DNA fragments according to their size

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Solution

The correct option is D
Separation of DNA fragments according to their size

Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.

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Q. How are the DNA fragments separated by gel electrophoresis is visualized and separated for use in constructing recombinant DNA?

Q. Select the correct option to fill up the blanks.

(i) _____ is a natural polymer extracted from ______.

(ii) The DNA fragments purified by gel electrophoresis are used in constructing _____ by joining them with ______.

(iii) The ligation of alien DNA is carried out at a _____ present in one of the two ____ in a plasmid vector.

(iv) _____ enzyme remains active during the high temperature-induced denaturation of ds DNA. DNA fragments are resolved according to their ______

(v) through _____ in agarose gel electrophoresis.

Q. Arrange the various steps of DNA fingerprinting technique in the correct order
(i) Separation of DNA fragments by electrophoresis
(ii) Digestion of DNA by restriction endonucleases
(iii) Hybridization using labeled VNTR probe
(iv) Isolation of DNA
(v) Detection of hybridized DNA fragments by autoradiography
(vi) Transferring the separated DNA fragments to nitrocellulose membrane

Q. Given below are the processes involved in recombinant DNA technology, which is used in the production of transgenic organisms:
1.Cutting of DNA at specific points to obtain the gene of interest
2.Isolation of DNA from the cellular components
3.Amplification of gene of interest
4.Inserting rDNA into the competent host cell
5.Inserting the gene of interest into a cloning vector
6.Separation of the isolated DNA fragments using agarose gel electrophoresis
From the options given below, choose the one which represents the correct order of these processes:

Name and explain techniques used in the separation and isolation of DNA fragments to be used in recombinant DNA technology?

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