Question

Gel used in gel fractionation chromatography is

• A. Agar
• B. Carrageenin
• C. Sephadex
• D. Acacia gel

Answer 1

Answer: (C)

Sephadex

• Gel fractionation chromatography is a technique of partition chromatography in which the partitioning is based on the molecular size of the substances to be separated.
• The principle involved in gel fractional chromatography is that the large particles cannot enter the gel and are excluded. They have less volume to transverse and elute sooner. Small particles can enter gel and have more volume to transverse. They elute later.
• The gel used in gel fractionation chromatography is sephadex.
• So, the correct answer is 'Sephadex'.