Question

Gene amplification requires besides sample DNA

• A. Nucleotides
• B. Nucleotides and polymerases
• C. Alternate heating and cooling
• D. Both B and C

Answer 1

The correct option is D Both B and C

Polymerase chain reaction (PCR) is an amplification technique for cloning the specific or targeted parts of a DNA sequence to generate thousands to millions of copies of DNA of interest. This molecular “xeroxing” process involves heating and cooling samples in a precise thermal cycling pattern over ≈30 cycles. PCR uses a small amount of template DNA, two primers that flank the target sequence, nucleotides, and thermostable DNA polymerase to amplify a specific region of DNA.

So, the correct answer is 'Both B and C.'