Question

Gene for controlling production of insulin is β-galactosidase. It can be used for (a) Blue white screening (b) PCR (c) Ladder (d)Gel electrophoresis

Answer 1

Option A is the correct one


The blue-white screen is a screening technique that allows for the detection of successful ligations in
vector-based gene cloning.





X-gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) is an organic compound consisting of galactose
linked to a substituted indole. X-gal is much used in molecular biology to test for the presence of an
enzyme, β-galactosidase. X-gal is one of many indoxyl glycosides and esters that yield insoluble blue
compounds similar to indigo as a result of enzyme-catalyzed hydrolysis in this case by β-
galactosidase.β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a
homotetramer in its active state.