The correct option is D 64 codons, each with three nucleotides
As it became evident that genes controlled the formation of polypeptides, it became more important to know how the four bases(A, T, C, G) in DNA lead to formation of 20 amino acids found in proteins.
Three nucleotides per codon can produce 43=64 combinations. This number is more than sufficient to code for 20 amino acids. Francis Crick, Sydney Brenner, and their colleagues provided genetic evidence for the triplet nature of code by studying mutagenic effects of a chemical on T4 bacteriophage.
The first elucidation of a codon was done by Marshall Nirenberg and Heinrich Matthaei in 1961.