Question

Genetic engineering is possible, because

• A. The phenomenon of transduction in bacteria is well understood
• B. We can see DNA by electron microscope
• C. We can cut DNA at specific sites by endonucleases like DNAase-I
• D. Restriction endonucleases purified from bacteria can be used in vitro

Answer 1

The correct option is D Restriction endonucleases purified from bacteria can be used in vitro

Genetic engineering is the manipulation of genetic material of an organism using enzyme restriction endonuclease.
Nathans and Smith (1970) isolated the first restriction endonuclease.
Jackson, Symons and Paul Berg (1972) successfully generated recombinant DNA molecules in vitro.

So, the correct answer is D 'Restriction endonucleases purified from bacteria can be used in vitro'