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Blunt and Sticky Ends

Genomic DNA i...

Question

Genomic DNA is digested with Alu I, a restriction enzyme which is a four base - pair cutter. What is the frequency with which it will cut the DNA assuming a random distribution of bases in the genome?

A

1/4

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B

1/24

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C

1/256

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D

1/1296

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Solution

The correct option is C
1/256

Alu I is a restriction endonuclease which is a 4 base pair cutter its frequency is 1/256 BP, while frequency of 6 base pair cutter Bam HI, EcoRI is 1/4096.

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