When a living cell, such as the cell of a leaf of an aquatic plant, is placed in freshwater, it remains in a fully distended condition. Its plasma membrane remains in close contact with the cell wall and presses against it just like a rubber bladder of a football pushing against the leather casing. If this plant cell is now kept in a hypertonic solution for 30 minutes, it will lose its distended appearance, the cytoplasm will shrink and the plasma membrane will withdraw from the cell wall, this is called plasmolysis and the cells in this state are said to be limp or flaccid.