Given below are a few diagrams with each representing a step in rDNA technology. Match the diagrams with the steps they represent.

i. Cloning
ii. Screening
iii. Gene incorporated in a vector
iv. Inserted into a host
v. Multiple copies using PCR
vi. Isolation of gene of interest

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Solution

The correct option is B
The steps in rDNA technology are as follows:
Step 1 - The desired gene, that is the ‘gene of interest’ is identified and isolated.
Step 2 - Multiple copies of the ‘gene of interest’ are made using techniques such as Polymerase Chain reaction.
Step 3 - Gene of interest is inserted into a delivery vehicle which will insert it into a host.
Step 4 - Gene of interest is delivered into a host living organism so that it can replicate and translate the protein of choice.
Step 5 - Screening to identify the organism that has received the rDNA.
Step 6 - Cloning of the organism containing the rDNA and extraction of the desired protein.

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Given below are a few diagrams with each representing a step in rDNA technology. Match the diagrams with the steps they represent. i. Cloning ii. Screening iii. Gene incorporated in a vector iv. Inserted into a host v. Multiple copies using PCR vi. Isolation of gene of interest

Given below are a few diagrams with each representing a step in rDNA technology. Match the diagrams with the steps they represent.

i. Cloning
ii. Screening
iii. Gene incorporated in a vector
iv. Inserted into a host
v. Multiple copies using PCR
vi. Isolation of gene of interest