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Question

Given below are the steps in the preparation of a temporary mount of a stained leaf peel.
(i) Cover the material with the cover slip
(ii) Transfer the stained peel to a clean glass slide and add a drop of glycerine
(iii) Remove the peel from lower surface of leaf
(iv) Drop it in water in Petri dish and add a drop of safranin stain.
The correct sequence of the experiment steps is

A
(a) (iii), (iv), (ii), (i)
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B
(b) (i), (ii), (iii), (iv)
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C
(c) (ii), (iii), (iv), (i)
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D
(d) (iii), (iv), (i), (ii)
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Solution

The correct option is A (a) (iii), (iv), (ii), (i)
A. (a) (iii), (iv), (ii), (i)
Solurtion : First the peel is removed from the lower surface of leaf. Then the peel is dropped in the petri dish of water and washed and drop of safranin is added as a stain. The the stained peel is transfered to clean glass slide and a drop of glycerine is added and a cover slip is placed over it.
So the correct answer is " (a) (iii), (iv), (ii), (i)".

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