Question

Given below are two statements: one is labelled as Assertion (A) and other is labelled as Reason (R).

Assertion (A) :
Polymerase chain reaction is used in $DNA$ amplification

Reason (R):
The ampicillin resistant gene is used as a selectable marker to check transformation

In the light of the above statements, choose the correct answer from the options given below:

• A. Both (A) and (R) are correct but (R) is not the correct explanation of (A)
• B. Both (A) and (R) are correct and (R) is the correct explanation of (A)
• C. (A) is correct but (R) is not correct
• D. (A) is not correct but (R) is correct

Answer 1

The correct option is A Both (A) and (R) are correct but (R) is not the correct explanation of (A)

• PCR or polymerase chain reaction is a technique used in molecular biology to create several copies of a certain DNA segment. A thermocycler or DNA amplifier is a laboratory device used to amplify small samples of DNA via polymerase chain reaction. PCR needs the reaction mixture to be taken through different levels of temperature very quickly for a successful amplification. $PCR$ enables the amplification of billions of copies of template $DNA$.
• Antibiotic resistance genes i.e. amplicillin resistant and tetracycline resistant gene act as selectable markers to distinguish between transformant and non transformant, recombinant and non recombinant.