Given below is the image depicting the steps involved in the manufacture of insulin using bacteria through genetic engineering. Match the numbers given in the image with the steps given below.
A. Joining the two DNA segments
B. Multiplication of bacteria
C. Identification of insulin gene
D. Insertion of the new genome into bacteria
E. Cutting the gene and bacterial genome
1 ⇒ C, 2 ⇒ E, 3⇒ A, 4⇒ D, 5⇒ B